周景文，男，1982.4.29，江南大学教授。研究领域：工业微生物代谢工程、工业微生物生理学。

，男，1982.4.29出生，职 称 教授。

以维生素C和苯丙素类化合物为目标产物，阐明微生物高效异源合成植物天然产物的生理机制，并通过计算机辅助的代谢途径设计、代谢途径单元模块化调控、辅因子平衡与再生调控、反义RNA工程等策略，在微生物中实现来源于植物的高附加值天然产物的高效异源合成。

前五年在Biotechnology Advances、Metabolic Engineering、Eukaryotic Cell和Applied and Environmental Microbiology等生物工程和微生物生理学权威学术期刊上发表与本项目相关的研究论文和综述45篇，授权发明专利14项，参编发酵工程专业著作4本。

代表性著作和论文如下:

[1] Saijie Zhu, Junjun Wu, Guocheng Du, Jingwen Zhou*, Jian Chen. Efficient synthesis of eriodictyol from L-tyrosine in Escherichia coli. Applied and Environmental Microbiology. 2014, 10(80): 3072-3080. (2012 IF= 3.678)

[2] Lili Gao, Yudong Hu, Jie Liu, Guocheng Du, Jingwen Zhou*, Jian Chen. Stepwise metabolic engineering of Gluconobacter oxydans WSH-003 for the direct production of 2-keto-L-gulonic acid from D-sorbitol. Metabolic Engineering. 2014, 24: 30–37. (2012 IF= 6.859)

[3] Shaohui Zhao, Xinrui Zhao, Huijun Zou, Jianwei Fu, Jian Chen, Jingwen Zhou, Guocheng Du. Comparative proteome analysis of Saccharomyces cerevisiae grown under different nitrogen source conditions. Journal of Proteomics. 2014, 101: 102-112(2012 IF=4.088)

[4] Jingwen Zhou, Guocheng Du, Jian Chen. Novel fermentation processes for manufacturing plant natural products. Current Opinion in Biotechnology. 2014. 25: 17-23.(2012 IF= 7.86)

[5] Xinrui Zhao, Guocheng Du, Huijun Zou, Jianwei Fu, Jingwen Zhou, Jian Chen. Metabolic engineering of the regulators in nitrogen catabolite repression to reduce the production of ethyl carbamate in a model rice wine system. Applied and Environmental Microbiology. 2014, 80(1): 392-398. (2012 IF= 3.678)

[6] Lili Gao, Jie Liu, Guocheng Du, Jingwen Zhou, Jian Chen. Characterization of a group of pyrroloquinoline quinone-dependent dehydrogenases that are involved in the conversion of L-sorbose to 2-Keto-L-gulonic acid in Ketogulonicigenium vulgare WSH-001. Biotechnology Progress. 2013. 29(6):1398-1404. (2012 IF= 1.853)

[7] Jidong Liu, Weiping Zhang, Guocheng Du, Jian Chen, Jingwen Zhou. Overproduction of geraniol by enhanced precursor supply in Saccharomyces cerevisiae. Journal of Biotechnology. 2013, 168(4): 446-451. (2012 IF= 3.183)

[8] Xinrui Zhao, Huijun Zou, Jianwei Fu, Jingwen Zhou, Jian Chen, Guocheng Du. Nitrogen regulation involved in the accumulation of urea in Saccharomyces cerevisiae used in rice wine production. Yeast. 2013. 30(11): 437-447.(2012 IF= 1.955)

[9] Junjun Wu, Peiran Liu, Yongming Fan, Han Bao, Guocheng Du, Jingwen Zhou, Jian Chen. Multivariate modular metabolic engineering of Escherichia coli to produce resveratrol from L-tyrosine. Journal of Biotechnology. 2013, 167: 404-411.(2012 IF= 3.183)

[10] Jingwen Zhou, Qiaoshuang Zheng, Jie Liu, Jianmin Huang, Guocheng Du, Jian Chen. Indigenous plasmids of Bacillus megaterium WSH-002 involved in mutualism with Ketogulonicigenium vulgare WSH-001. Plasmid. 2013, 70(2):240-246. (2012 IF=1.276)

[11] Xinrui Zhao, Guocheng Du, Huijun Zou, Jianwei Fu, Jingwen Zhou*, Jian Chen. Progress in preventing the accumulation of ethyl carbamate in alcoholic beverages. Trends in Food Science and Technology. 2013, 32: 97-107.(2012 IF=4.135)

[12] Xiaobei Wang, Jie Liu, Guocheng Du, Jingwen Zhou, Jian Chen. Efficient production of L-sorbose from D-sorbitol by whole cell immobilization of Gluconobacter oxydans WSH-003. Biochemical Engineering Journal. 2013, 77: 171–176.(2012 IF= 2.579)

[13] Jidong Liu, Yibo Zhu, Guocheng Du, Jingwen Zhou, Jian Chen. Response of Saccharomyces cerevisiae to D-limonene-induced oxidative stress. Applied Microbiology and Biotechnology. 2013, 97(14): 6467-6475. (2012 IF=3.689)

[14] Yongkun Lv, Xinrui Zhao, Long Liu, Guocheng Du, Jingwen Zhou*, Jian Chen. A simple procedure for protein ubiquitination detection in Saccharomyces cerevisiae: Gap1p as an example. Journal of Microbiological Methods. 2013, 94: 25-29. (2012 IF=2.161)

[15] Jingwen Zhou, Zhenghong Xu, Shouwen Chen. Simulation and prediction of the thuringiensin abiotic degradation processes in aqueous solution by a radius basis function neural network model. Chemosphpere. 2013. 91(4): 442-447. (2012 IF=3.137)

[16] Shimon Bershtein, Wanmeng Mu, Adrian Serohijos, Jingwen Zhou, Eugene I. Shakhnovich. Protein quality control shapes the fitness effects of mutations in E. coli. Molecular Cell. 2013. 49(1): 133-144. (2012 IF=15.28)

[17] Jidong Liu, Yibo Zhu, Guocheng Du, Jingwen Zhou, Jian Chen. Exogenous ergosterol protects Saccharomyces cerevisiae from D-Limonene stress. Journal of Applied Microbiology. 2013. 114(2):482-491.(2012 IF=2.196)

[18] Junjun Wu, Guocheng Du, Jingwen Zhou, Jian Chen. Metabolic engineering of Escherichia coli for (2S)-pinocembrin production from glucose by a modular metabolic strategy. Metabolic Engineering. 2013. 16:48-55.(2012 IF= 6.859)

[19] Hua Liu, Guocheng Du, Jianghua Li, Jingwen Zhou, Jian Chen. Enhanced production of a-cyclodextrin glycosyltransferase in Escherichia coli by codon usage optimization. Journal of Industrial Microbiology & Biotechnology. 2012. 39(12): 1841-1849. (2012 IF=2.321)

[20] Xiaoxia Yin, Catherine Madzak, Guocheng Du, Jingwen Zhou, Jian Chen. Enhanced a-ketoglutarate production in Yarrowia lipolytica WSH-Z06 by regulation of the pyruvate carboxylation pathway.Applied Microbiology and Biotechnology. 2012. 96(6): 1527-1537. (2012 IF=3.689)

[21] Jingwen Zhou, Hua Liu, Jianghua Li, Guocheng Du, Jian Chen. Production of a-cyclodextrin glycosyltransferase in Bacillus megaterium MS941 by systematic codon usage optimization. Journal of Agricultural and Food Chemistry. 2012, 60(41), 10285–10292. (2012 IF=2.906)

[22] Jingwen Zhou, Xiaoxia Yin, Catherine Madzak, Guocheng Du, Jian Chen. Enhanced a-ketoglutarate production in Yarrowia lipolytica WSH-Z06 by alteration of the acetyl-CoA metabolism. Journal of Biotechnology. 2012.161: 257-264. (2012 IF= 3.183)

[23] Feifei Hu, Jidong Liu, Guocheng Du, Zhaozhe Hua, Jingwen Zhou, Jian Chen. The key cytomembrane ABC transporters of Saccharomyces cerevisiae failed to improve the tolerance to D-limonene. Biotechnology Letters. 2012. 34(8): 1505-1509. (2012 IF= 1.853)

[24] Lili Gao, Jingwen Zhou, Jie Liu, Guocheng Du, Jian Chen. Draft Genome Sequence of Gluconobacter oxydans WSH-003, an Extremely Saccharide and Alditol-Tolerant Strain. Journal of Bacteriology. 2012. 194(16) 4455-4456. (2012 IF=3.194)

[25] Yibo Zhu, Jie Liu, Guocheng Du, Jingwen Zhou, Jian Chen. A high throughput method to screen companion bacterium for 2-keto-L-gulonic acid biosynthesis by co-culture Ketogulonigenium vulgare. Process Biochemistry. 2012. 47: 1428-1432. (2012 IF=2.414).

[26] Zongzhong Yu, Guocheng Du, Jingwen Zhou, Jian Chen. Enhanced a-ketoglutaric acid production in Yarrowia lipolytica WSH-Z06by an improved integrated fed-batch strategy. Bioresource Technology. 2012. 114: 597-602. (2012 IF=4.75)

[27] Yibo Zhu, Jie Liu, Guocheng Du, Jingwen Zhou, Jian Chen. Sporulation and stable spore of Bacillus megateriumenhance Ketogulonigenium vulgarepropagation and 2-keto-L-gulonic acidbiosynthesis. Bioresource Technology. 2012. 107: 399-404.(2012 IF=4.75)

[28] Zhengxiong Zhou, Guocheng Du, Zhaozhe Hua, Jingwen Zhou, Jian Chen. Optimization of fumaric acid production by Rhizopus delemar based on the morphology formation. Bioresource Technology. 2011, 102: 9345-9349. (2012 IF=4.75)

[29] Jingwen Zhou, Liming Liu, Jian Chen. Improved ATP supply enhances acid tolerance ofCandida glabrata during pyruvic acid production. Journal of Applied Microbiology. 2011, 110(1):44-53. (2012 IF=2.196)

[30] Jingwen Zhou, Liming Liu, Jian Chen. Method to purify mitochondrial DNA directly from yeast total DNA. Plasmid. 2010, 64(3):196-199. (2012 IF=1.276)

[31] Jingwen Zhou, Haiyan Zhou, Guocheng Du, Liming Liu, Jian Chen. Screening of a thiamine auxotrophic yeast for α-ketoglutaric acid overproduction. Letters in Applied Microbiology. 2010, 51(3):264-271.(2012 IF=1.629)

[32] Jingwen Zhou, Liming Liu, Jian Chen. Mitochondrial DNA heteroplasmy in Candida glabrata after mitochondrial transformation. Eukaryotic Cell. 2010, 9(5): 806-814. (2012 IF=3.586)

[33] Jingwen Zhou, Liming Liu, Zhongping Shi, Guocheng Du, Jian Chen. ATP in Current Biotechnology: Regulation, Application and Perspectives. Biotechnology Advances. 2009, 27(1): 94-101. (2012 IF= 9.599)

[34] Jingwen Zhou, Luxi Huang, Liming Liu Jian Chen. Enhancement of pyruvate productivity by inducible expression of a F0F1-ATPase inhibitor INH1 in Torulopsis glabrata CCTCC M202019. Journal of Biotechnology. 2009, 144(2): 120-126. (2012 IF= 3.183)

[35] Jingwen Zhou, Zhiyao Dong, Liming Liu, Guocheng Du, Jian Chen. A reusable method for construction of non-marker large fragment deletion yeast auxotroph strains: a practice inTorulopsis glabrata. Journal of Microbiological Methods. 2009, 76(1): 70–74. (2012 IF=2.161)

[36] Jingwen Zhou, Yafei Chang, Zhenghong Xu, Ziniu Yu, Shouwen Chen. Production of thuringiensin by fed-batch culture of Bacillus thuringiensis subsp. darmstadiensis 032 with an improved pH-control glucose feeding strategy, Process Biochemistry. 2007, 42(1): 52-56.(2012 IF=2.414)

[37] 郭洪伟, 堵国成, 陈坚, 周景文*. 利用微生物发酵生产a-酮戊二酸进展. 生物工程学报. 2013, 29(2): 141−152.

[38] 孙明雪, 刘继栋, 堵国成, 周景文*, 陈坚. 调控酿酒酵母类异戊二烯合成途径强化芳樟醇合成. 生物工程学报. 2013, 29(6): 751−759.

[39] 殷晓霞, 堵国成, 陈坚, 周景文*. 强化乙酰辅酶A供给对解脂亚洛酵母产a-酮戊二酸的影响. 工业微生物. 2013, 43(2): 45-50.

[40] 余宗钟, 堵国成, 陈坚, 周景文*. 基于细胞代谢调控的a-酮戊二酸发酵过程放大. 工业微生物. 2013, 43(3): 23-28.

[41] 周景文, 董志姚, 刘立明, 堵国成, 陈坚. 葡萄糖酸钠对光滑球拟酵母CCTCC M202019能量代谢和丙酮酸积累的影响. 华中农业大学学报(自然科学版), 2010, 29(4): 10-15.

[42] 周景文, 徐建, 陈守文, 喻子牛. 基于径向基神经网络和遗传算法的聚-γ-谷氨酸发酵培养基优化. 食品科学. 2006, 27(10): 270-274.

[43] 周景文，堵国成，陈坚*. 发酵食品有害氨(胺)类代谢物：形成机制和消除策略. 中国食品学报. 2011, 9: 8-25.

[44] 朱益波, 周景文*，陈坚. 巨大芽孢杆菌与普通生酮基古龙酸菌共培养过程中相互作用分子机制研究进展. 微生物学报. 2012, 52(8):940-947.

[45] 张伟平, 赵鑫锐, 堵国成, 邹慧君, 傅建伟, 周景文*, 陈坚. 酿酒酵母氮代谢物阻遏效应及其对发酵食品安全的影响. 应用与环境生物技术学报. 2012, 18(05): 862-872.

*: 通讯作者。

前五年主持或完成国家自然科学基金、江苏省自然科学基金和国家/部级重点实验室开放课题及企业横向委托项目8项。其中代表性的科研项目如下:

[1] 解脂亚洛酵母酮酸转运蛋白的生理功能与调控机制. 国家自然科学基金面上项目(21276109). 2013年1月1日-2016年12月1日. 项目主持人；

[2] 微生物高效积累黄酮类化合物的关键共性问题研究. 江南大学自主科研计划-重点项目(JUSRP51307A), 2013年1月-2015年12月. 项目主持人；

[3] 维生素C合成过程吡咯喹啉醌代谢关键基因及其调控机制. 教育部新世纪优秀人才支持计划(NCET-12-0876), 2012年1月-2015年12月. 项目主持人；

[4] 维生素C从头合成关键模块功能解析与调控，高等学校全国优秀博士学位论文作者专项资金(2011046)，2012年1月-2016年12月. 项目主持人；

[5] 生化网络重构与维生素生产新菌种. 863项目(2012AA022103).2012年11月1日-2015年12月31日；

[6] 食品安全全程控制的食品制造过程设计与构建. 国家重点基础研究发展计划(973计划，2012CB720806). 2012年1月-2016年8月. 子课题负责人；

[7] 代谢工程改造酿酒酵母合成D-柠檬烯的关键问题研究. 国家自然科学基金青年科学基金项目 (31000807). 2011年1月1日-2013年12月1日. 项目主持人；

[8] 代谢工程改造酿酒酵母合成S-芳樟醇及其发酵过程优化. 江苏省基础研究计划(BK201020722). 2010年 7月1日-2013年7月1日. 项目主持人。

[1] 2013年12月，新型有机酸的发酵法制备技术，陈坚、周景文、刘龙、李江华、刘立明，2013年中国轻工业联合会技术发明奖一等奖；

[2] 2012年8月，发酵法生产α-酮戊二酸，堵国成、周景文、李江华、陈坚、宗伟刚、胡志杰、王立军，2012年无锡市科技进步奖二等奖；

[3] 2011年11月，光滑球拟酵母中ATP的生理功能与作用机制，周景文，全国百篇优秀博士学位论文(证书编号：2011046)；

[4] 2011年10月，《发酵过程优化原理与技术》，陈坚、堵国成、刘立明、周景文、刘龙等，中国石油和化学工业联合会，科技进步奖一等奖(证书编号：2011JBR0516-1-6)；

[5] 2011年2月，工业微生物生理特性与代谢功能研究，陈坚、堵国成、刘立明、吴敬、周景文、张娟、刘龙、张东旭，江苏省科技技术奖一等奖(证书编号：2010-1-3-R-5)；

[6] 2010年10月，光滑球拟酵母中ATP的生理功能与作用机制，周景文，江苏省2010年度优秀博士论文(证书编号：CX10B064)。